1. Online purchase: A subscription purchased online is activated immediately in mybiocrates. To check your subscription status, log in to my.biocrates.com.

2. Voucher (offline purchase): A subscription purchased via voucher can be activated by the end user at any time within the 30-day activation period. After this period, the voucher code expires and can no longer be used. In such cases, please contact support@biocrates.com to request a new code. The start date of the subscription will be set to the last day of the original activation period.

There is a general market transition from traditional on-premises software to cloud-based services. Many leading software providers have shifted their portfolio to the cloud (e.g. Microsoft, Oracle, SAP, Google) as benefits exceed the disadvantages. In case your institutional restrictions apply, you may use the on-premises subscription, but in many cases an additional agreement (DPA) enables the use of our cloud service (Please consult your legal department!).
You can always migrate from on-premises to cloud-based service any time.

WebIDQ client requirements | cloud and on-premises​

WebIDQ server requirements | on-premises only​

Hardware where the database and WebIDQ installation are hosted.

For the installation and maintainance local administrative access rights are necessary. To install and maintain WebIDQ on-premises it is mandatory to involve your IT specialists!

Yes, WebIDQ includes a migration wizard and online instructions that help to easily move MetIDQ data to WebIDQ. Our customer support team will be there for you should you need any assistance.

WebIDQ is a multi user application. The total amount of users who can access the software concurrently depends on the subscription plan. Please lookup the details on the product detail pages.

biocrates is committed to protecting and honoring your privacy rights. We fully comply with the GDPR regulations. Each organization gets their own secure and unique database instance assigned for their data. It is not possible to access other organization’s data. All client-to-service communication is encrypted (SSL protection) and access within the organization is managed by user role management through the organizations’ administrators.

All data of biocrates cloud applications, i.e. biocrates webshop, mybiocrates, WebIDQ cloud and QMDB, are stored in European data centers. We are fully GDPR compliant. Please refer to our Terms & Conditions and Privacy Policy for details.

QMDB
After expiration of the subscription access will not be possible anymore. After some time of inactivity the account will be marked for deletion.

WebIDQ
For 30 days a read-only mode will be activated with following impact to the service

• WebIDQ start button will not be available in mybiocrates
• WebIDQ can only be accessed through the URL https://webidq.biocrates.com/
• The download section will also not be available in mybiocrates
• Only LIMS and Results module will be available
• Export of raw data files and result files is possible

1. Uploaded raw data files (e.g. ".wiff" files) are converted to mzML format and these are stored in the WebIDQ database.
   Please note: The original files are not stored in WebIDQ. Please consider an appropriate backup!
   
2. The WebIDQ database, including the mzML files, is regularly backed up by biocrates. Following retention policy is applied: 

   Month	- 1	- 2	- 3	- 4	- 5	- 6	- 7	- 8	- 9	- 10	- 11	- 12
   frequency of backup	daily	every other day	weekly	weekly	weekly	biweekly	biweekly	biweekly	monthly	monthly	monthly	monthly

3. Four months after the term for WebIDQ Cloud has ended the Client Data in the storage will be deleted.
   
   
4. When WebIDQ on-premises expires, access is restricted. However, data is not deleted.

In addition to your mass spectrometer operating software (e.g. Analyst, MassHunter, MassLynx, Xcalibur), you need the WebIDQ workflow management solution, which is available as a cloud or on-premises subscription separate from the kits: WebIDQ cloud core

WebIDQ contains tools for data normalization and analysis as well as MetaboINDICATOR.
Additionally, biocrates offers a statistical analysis service and interpretation package performed by our in-house experts.

The lifespan of the column depends heavily on the number and type of samples being run, as well as the storage conditions between plates (column should be stored in pure LC-MS grade acetonitrile). Running plasma samples, the column system can normally be used for 15-20 kits (1200-1600 samples, MxP® Quant 500). Dirtier sample matrices (e.g. urine or fecal samples) may reduce the lifespan. Usually a minimum of 10 kits (800 samples, MxP® Quant 500) can be run per column irrespective of sample type. Regardless of the number of runs, chromatography should always be evaluated immediately after every LC run. As the column ages, the early eluting peaks will begin to broaden and show peak fronting, shoulder peaks, or in worse cases double peaks. Retention times of the early eluting compounds may also begin shifting earlier. Late eluting peaks are not as affected by column age. Some of these issues may also be a result of a leak somewhere in the system. Loosen and retighten all fittings and perform test injections before replacing the column.

If the peaks becoming jagged, it may be a result of instability in the electrospray (ESI electrode, gas flow, contamination, etc.) rather than chromatography related.

Please download the respective kit requirements document for the product you want to buy. The full mass spectrometry system compatibility list can be found here.

MxP® Quant 1000 kit

MxQuant kit 

LxQuant kit

MxP® Quant500 XL 

MxP® Quant500  

AbsoluteIDQ® p400 HR kit

AbsoluteIDQ® p180 kit

AbsoluteIDQ® Bile Acids kit

MxP®Quant HR Xpress kit

The Stero17 kit requires 250-500 µL of plasma or serum, depending on the used mass spectrometer (whereas only serum has been validated for that kit). All other kits require 10 µL of plasma or serum. For other matrices or further details, please contact biocrates support.

Run time per sample / min

Run time per kit / h

Expiration date for Quant 500 and Quant 500 XL kits:

• Storage at -20°C: 3 months after shipping date
• Storage at -80°C: 12 months after shipping date

Expiration date for Quant 1000, MxQuant and LxQuant kits:

• Storage at -80°C: 6 months after shipping date

Expiration date for p180, p400 HR, Quant HR Xpress and Bile Acids kits:

• Storage at -20°C: 6 months after shipping date
• Storage at -80°C: 12 months after shipping date

• • Plate can be run within 48h: Store plates at 4°C
  • Plate cannot be run within 48h: Contact biocrates support!

biocrates kits have been specifically validated for use with plasma and serum. However, a wide variety of other sample matrices can be analyzed using the kits. Essentially any samples with endogenous metabolites can be analyzed with the correct preparation.
For most kits, protocols for the following matrices are available:

• Tissue
• Urine
• Fecal
• Cell culture
• Dried blood spots
• Cerebrospinal fluid

If you are interested in other matrices, please contact biocrates support!

A kit must be prepared all at once, as it is sealed under nitrogen to prevent degradation. Kits are for single use only.

biocrates kits have been specifically validated for use with plasma and serum. Feasibility tests have been carried out with other sample matrices and protocols are available (see below). Basically, any samples containing endogenous metabolites can be analyzed with the correct preparation. However, for any samples other than plasma or serum, we always recommend performing pilot tests with representative samples before starting a larger study. The results may depend on the nature and on the quality of the samples.

For most kits, protocols for the following matrices are available:
- Tissues
- Urine
- Cells
- Dried blood spots (DBS)
- Cerebrospinal fluid (CSF)

Kits were validated and tested on specific MS instruments so that we can provide all optimized methods to our users and guarantee the high level of performance you can expect.

biocrates kits were validated with EDTA and heparin plasma only. Citrate plasma may also be used, but performance may be reduced. The most important aspect is to ensure that all plasma samples within one study are collected in the same way and using the same anticoagulant. For a summary of various anticoagulants and their effect on metabolomics samples, please refer to this peer-reviewed study.

• Any UHPLC or HPLC system will work, as long as the minimum specifications of the kit requirements are met
• Autosampler: Any model works that supports 96 well plates, cooling, and a column oven
• An experienced instrument operator is required to make sure that configurations and communications work between LC and MS systems.

While the biocrates kits come with all the standards and quality controls necessary, some other additional solvents and lab equipment are required to successfully prepare and run a plate. Please contact biocrates support for a detailed list of requirements and recommendations.

You can run the kits in our certified partner laboratories worldwide. Moreover, we offer sample analysis and statistical analysis up to data interpretation through our metabolic phenotyping services, . Please contact us for more information: https://shop.biocrates.com/Services/.