For AbsoluteIDQ® p180, p400 HR and MxP® Quant 500 kits

When adding water to the methanolic sample extracts to make the LC plate, cloudiness can occur due to residues of pyridine and phenyl isothiocyanate from the derivatization solution. If that happened, perform the following steps:

1. Extend the shaking time at 600 rpm to 20-30 min.
2. If the extracts are still cloudy, leave the plate standing for 15 min at room temperature and shake for another 15 min at 600 rpm.
3. Afterwards, run the plate, even if the extracts are still a bit cloudy. We found that the cloudiness has no impact on the kit performance or on the quality of the data. Also, we did not observe any wastage of hardware parts.