AbsoluteIDQ® Bile Acids kit

Product number: 20813.5
LC system
Instrument compatibility
Ready-to use_Kitbox kw


Data collection_kreisweiss

Standardized and quantitative

Simple Guided Workflow_Zahnräder

Guided workflow

High Throughput Uhr

High throughput with minimal sample volume

Kit components

Reagents and consumables

  • Patented 96-well filter plate
  • Internal and calibration standards
  • Quality controls
  • Testmix

Methods and protocols

  • Sample preparation protocol
  • Instrument-specific acquisition and quantification methods
  • System suitability test

Kit requirements

  • WebIDQ - Workflow manager
  • AbsoluteIDQ® Bile Acids UHPLC column

Metabolite coverage

  • 10 primary bile acids: produced by the liver (CA, GCA, TCA, CDCA, GCDCA, TCDCA, α-MCA, β-MCA, ω-MCA, TMCA(α+β)
  • 10 secondary bile acids: produced by gut bacteria from primary bile acids (DCA, GDCA, TDCA, LCA, GLCA, TLCA, UDCA, GUDCA, TUDCA, HDCA)

AbsoluteIDQ® Bile Acids kit

The benchmark solution to assess host-microbiota interaction

Bile acids are produced in the liver and are best known to facilitate the absorption of dietary fats and fat-soluble vitamins. Beyond this, bile acids exert important signaling and regulatory functions via nuclear receptors such as the farnesoid X receptor (FXR). Since gut bacteria convert primary into secondary bile acids, they are essential to study the role of gut microbiota in health and disease. The AbsoluteIDQ Bile Acids kit is the first ready-to-use, standardized solution for the quantitation of 20 bile acids by liquid chromatography-mass spectrometry (LC-MS).

Expiration date 

  • 6 months after shipping date
  •     when stored at -20 °C
  • 12 months after shipping date
  •     when stored at -80 °C

For research use only. Not for use in diagnostic procedures.